Lajjalu (Mimosa Pudica)

Recent Research on Lajjalu (Mimosa pudica)

• Sutar, Nitin & Sutar, U & Behera, B. (2009). Antidiabetic activity of the leaves of Mimosa pudica Linn. in albino rats. Journal of Herbal Medicine and Toxicology. In the present study, attempts were made to study the anti-diabetic activity of the leaves of Mimosa pudica Linn belonging to the family Mimosaceae. Ethanolic and Petroleum ether extract of Mimosa pudica Linn were used and compared with Metformin as a standard drug (500 mg/ kg). Wister strain of either sex was treated with Alloxan (150 mg/ kg) to induce diabetes. The Glucose Oxidase/ Peroxidase method was used for the determination of plasma glucose level. The ethanolic extract showed a significant decrease in blood glucose levels.
• J, Sneha & Masalekar, Srinivas & D, Chandrakala. (2024). A conceptual study on the efficacy of Lajjalu Moola Taila in Sadhyovrana. Journal of Ayurveda and Integrated Medical Sciences. 9. 245- 249. 10. 21760/ jaims. 9. 2. 37. A surgeon’s initial challenge is the management of Vrana (wound). Vrana is the one that does the Gatravichurnana (disruption of tissues) and produces Vivarnata (discoloration) of the defective body part. Sadhyovranas are those which are caused by trauma and exogenous factors. Sadhyo Vrana encompasses various types of fresh wounds, including traumatic wounds, burn wounds, animal bites, and post-operative wounds. The aim of wound care is to promote wound healing in the shortest time possible, with minimal pain and scarring. While the promotion of wound healing remains a topic of discussion in modern science, the ancient system of Ayurveda has described the concept of Vrana Ropana and is elaborated in the treatise Sushruta Samhita under Shashti Upakrama.[1] There is a need to explore this unique concept of Vrana Ropana under which many formulations have been mentioned in various classics which aid in the early healing of the wounds. In the context of Sadhyovrana, Acharya Shodala, as cited in the Gadanigraha, refers to the use of Lajjalumoolataila especially in shastraghata Vrana. He particularly emphasizes its Ropanakarma function, which accelerates wound healing by preventing Paka, thus, ensuring a quick recovery. Hence, an effort has been undertaken to explore the effectiveness of Lajjalumoola Taila in promoting wound healing, with a particular focus on its application in Sadhyovrana.
• Singha, Rishov & Kanthal, Lakshmi & Pattanayak, Suman & Maiti, Mouli & Bhuniya, Tirthankar & Maity, Prithwiraj. (2023). In-vitro Anti-inflammatory Activity of Mimosa pudica Against Inhibition of Protein Denaturation and Heat-induced Haemolysis Methods. International Journal of Pharmaceutical Sciences Review and Research. 82. 10. 47583/ ijpsrr. 2023. v82i01. 008.
• Joseph, Baby & George, Jency & Mohan, Jeevitha. (2013). Pharmacology and Traditional Uses of Mimosa pudica. International Journal of Pharmaceutical Sciences and Drug Research. 5. 41- 44. Mimosa belongs to the taxonomic group Magnoliopsida and family Mimosaseae. In Latin it is called Mimosa pudica Linn. Ayurveda has declared that its root is bitter, acrid, cooling, vulnerable, and alexipharmic. It is used in the treatment of leprosy, dysentery, vaginal and uterine complaints, inflammations, burning sensation, asthma, leucoderma, fatigue, and blood diseases. Decoction of the root is used as a gargle to reduce toothache. It is very useful in diarrhea (atisaara), amoebic dysentery (Raktaatisaara), bleeding piles, and urinary infections. 
• Rajendiran, Deepa & Kandaswamy, Dr & Sivanesan, Senthilkumar & Radhakrishnan, Saravanan & Gunasekaran, Krishnamoorthy. (2017). Potential Antidiabetic Effect of Mimosa pudica Leaves Extract in High Fat Diet and Low Dose Streptozotocin-induced Type 2 Diabetic Rats. Mimosa pudica (Mp) is a traditional plant, whose (leaves, roots, and whole plant of Mp) are used for the treatment of diabetes mellitus (DM). In Siddha medicine, Mp Chooranam is prepared and used for the treatment of type 2 DM. The present study was designed to evaluate the antidiabetic effect of Mp leaf extract on key enzymes of carbohydrate metabolic enzyme activities in control and streptozotocin (STZ) induced diabetic rats. Diabetes was induced by feeding a high-fat diet (HFD) for 2 weeks followed by intraperitoneal injection of streptozotocin (35 mg/ kg bwt). Seven days after STZ injection, diabetic rats were supplemented with ethanolic Mp leaf extract (300 mg/ kg) daily for 30 days. The effect of ethanolic Mp leaf extract on blood glucose, plasma insulin, glycosylated hemoglobin, and glucose metabolizing enzymes of Type 2 diabetic rats was studied. The ethanol extract of Mp leaves showed a significant effect of antihyperglycemic activity by altering carbohydrate metabolizing enzyme activity and insulin secretion.
• Vikram, Pradeep & Malvi, Reetesh & Jain, Deepak. (2015). EVALUATION OF ANALGESIC AND ANTI-INFLAMMATORY POTENTIAL OF MIMOSA PUDICA LINN. International Journal of Current Pharmaceutical Research. This work aims to evaluate the acute toxicity, Analgesic and Anti-Inflammatory activity of Ethanolic extract of Mimosa Pudica Linn. In the acute toxicity study, the extracts were administered in doses of 5, 50, 300, and 2000 mg/ kg p. o., and behavioral changes were observed after 24 hrs. In the hot plate test the pethidine-treated group, Tail flick Diclofenac treated group, and group given ethanolic extracts as 250 mg/ kg and 500 mg/ kg showed an increase in latency time dose-dependent manner. Oral administration of ethanolic extract at a dose of 500 mg/ kg showed a significant reduction of writhing response induced by acetic acid as compared to the dose given at 250 mg/ kg.
• Joseph, Alex & Mathew, jesil & m, athulya. (2008). Antioxidant & anti diabetic activity of mimosa pudica Linn. in streptozotocin induced diabetic rats. Biomed.
• Parasuraman, Subramani & Ching, Teoh & Leong, Chong & Banik, Urmila. (2019). Antidiabetic and antihyperlipidemic effects of a methanolic extract of Mimosa pudica (Fabaceae) in diabetic rats. Egyptian Journal of Basic and Applied Sciences. 6. 1- 12. 10. 1080/ 2314808X. 2019. 1681660. Mimosa pudica (Fabaceae) is a perennial herb and this plant is reported to have anticonvulsant, antimicrobial, antioxidant, antiulcer, antiasthmatic, and wound healing activities. The diabetes dyslipidemic effect of Mimosa pudica is not clear. Hence the study is planned to investigate the antidiabetic and antihyperlipidemic activities of methanolic extract of Mimosa pudica (MEMP) on streptozotocin-induced diabetes mellitus in Sprague-Dawley rats. Antidiabetic and antihyperlipidemic effects of a MEMP were studied at 125, 250, and 500 mg/ kg body weight (BW). The antidiabetic potential of MEMP was compared with glibenclamide 20 mg/ kg BW. The body weight of rats and blood glucose levels were monitored at regular intervals during the experiment. At the end of the study, the blood sample was collected from the rats for biochemical analysis, and they were sacrificed, and their organs were used for histopathological analysis. Throughout the study, the diabetic control rats showed a significant increase in glucose level, total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL) and very-low-density lipoprotein (VLDL) when compared with that of control, whereas the animals treated with glibenclamide and MEMP showed a significant reduction in the levels of glucose, TG, LDL, and VLDL when compared with that of diabetic control. In conclusion, MEMP showed significant antidiabetic and antihyperlipidemic activities in streptozotocin-induced diabetic rats.
• Subramani, Meenatchisundaram & Michael, Antonysamy. (2009). Preliminary studies on antivenom activity of Mimosa pudica root extracts against Russell’s viper and saw scaled viper venom by in vivo and in vitro methods. Pharmacologyonline. 2. 372- 378. Mimosa pudica aqueous root extracts were tested for their inhibitory activity on various pharmacological effects like lethality, phospholipase activity, edema forming activity, fibrinolytic activity, and hemorrhagic activity of Russell’s viper and saw scaled viper venoms. The aqueous extract displayed a significant inhibitory effect on lethality, phospholipase activity, edema-forming activity, fibrinolytic activity, and hemorrhagic activity. About 0.13 mg and 0.17 mg of Mimosa pudica plant extracts were able to completely neutralize the lethal activity of 2LD50 of Russell’s viper and saw scaled viper venoms respectively. The present finding suggests that aqueous extracts of M. pudica root possess compounds, which inhibit the activity of Russell’s viper and saw-scaled viper venoms.
• Tunna, Tasnuva & Ahmed, Qamar & Helal Uddin, A. B. M. & Sarker, Md Zaidul. (2014). Weeds as Alternative Useful Medicinal Source: Mimosa pudica Linn. on Diabetes Mellitus and its Complications. Advanced Materials Research. 995. 49- 59. 10. 4028/ www. scientific. Net/ AMR. 995. 49. Diabetes mellitus is one of the major reasons for mortality worldwide and numerous scientific studies are going on to find plausible solutions to overcome and manage diabetes and its related infirmities. Traditional medicines use medicinal plants as anti-diabetic agents and despite being a disturbing weed to farming land Mimosa pudica Linn. has a high traditional usage for various purposes including anti-diabetic complications. The objective of this article is to accumulate and organize literature based on traditional claims and correlate those with current findings on the use of M. pudica in the management of diabetes mellitus. M. pudica is a creeping perennial shrub that is a common weed widely distributed in Southeast Asia, especially in India, Bangladesh, Malaysia, China, the Philippines, etc. This plant has various species of which M. pudica is a well-recognized plant of medicinal origin that has been traditionally used as folk medicine in India, Bangladesh, and Philippines, Chinese, herbal, and Siddha medicines. It has wound healing, antidiabetic, anti-diarrhoeal, antimicrobial, anti-cancer, anti-infection, anti-worm, anti-proliferative, anti-snake venom, anti-depressant and anxiolytic, etc. activities. The objective of this article is to provide up-to-date information on the traditional and scientific studies based on this plant on the frontier of diabetes mellitus. The methodology followed was to methodically collect, organize, and chart the recent advances in the use of M. pudica in diabetes and its related complications like vascular complications, diabetic wounds, hyperlipidemia, etc. Various scientific studies and traditional literature clearly support the use of M. pudica as an anti-diabetic agent among other uses. So far, the anti-diabetic compounds have not been isolated from this plant and this can be a good scientific study for the future anti-diabetic implications.
• Prakasam, N.Vishal & Devi, Gayatri & Jayaraman, Selvaraj & Priya, Jothi. (2022). In-vitro Antidiabetic on leaf extracts of Mimosa pudica and Euphorbia hirta – A Comparative Study. Research Journal of Pharmacy and Technology. 5459- 5463. 10. 52711/ 0974- 360X. 2022. 00920. Background: Mimosa pudica commonly known as the “Touch me not” originated from the family “mimosaceae”. Mimosa pudica possesses a lot of medicinal values such as anti-bacterial, anti-fungal, and anti-diabetic properties, etc. Euphorbia hirta is commonly known for curing patients suffering from asthma hence the name “Asthma plant”. It has various medicinal properties such as anti-fungal,anti-bacterial,anti-diabetic, etc. Both these plants have anti-diabetic properties hence the present was done to compare which medicinal herb is a better replacement for the standard synthetic drug “Metformin”. Materials and Methods: Assessment of in vitro anti-diabetic activity of Mimosa pudica and Euphorbia hirta was performed by alpha-amylase inhibitory activity and alpha-glucosidase inhibitory activity. The result was compared to the standard drug metformin. The data were analyzed statistically using a one-way analysis of variance (ONE-WAY ANOVA). Duncan’s Multiple range test was used to analyze the statistical significance between groups. The levels of significance were considered at the levels of p< 0.05. Result: Both the plants showed an increase in the percentage of inhibition of alpha-amylase and alpha-glucosidase in dose dose-dependent manner. Conclusion: In the present study, it was revealed that the medicinal plant Euphorbia hirta showed better antidiabetic activity than Mimosa pudica.
• Subramani, Meenatchisundaram & Priyagrace, Selvin & Vijayarghavan, Ramasamy & Velmurugan, Ambikapathi & Govindarajan, Parameswari & Michael, Antonysamy. (2009). Antitoxin activity of Mimosa pudica root extracts against Naja naja and Bangarus calculus venoms. Bangladesh Journal of Pharmacology. 4. 10. 3329/ bjp. v4i2. 2276. The aqueous extract of dried roots of Mimosa pudica was tested for inhibitory activity on lethality, phospholipase activity, edema forming activity, fibrinolytic activity, and hemorrhagic activity of Naja naja and Bangarus caerulus venoms. The aqueous extract displayed a significant inhibitory effect on lethality, phospholipase activity, edema-forming activity, fibrinolytic activity, and hemorrhagic activity. About 0.14 mg and 0.16 mg of M. pudica extracts were able to completely neutralize the lethal activity of 2LD50 of Naja naja and Bangaru’s calculus venoms respectively. The present finding suggests that aqueous extract of M. pudica root possesses compounds, which inhibit the activity of Naja naja and Bangarus caerulus venoms.
• Amalraj, T & Ignacimuthu, Savarimuthu. (2002). Hyperglycemic effect of leaves of Mimosa pudica Linn. Fitoterapia. 73. 351- 2. 10. 1016/ S0367- 326X (02) 00079- 5. Ethanolic extract of Mimosa pudica leaves given by oral route to mice at a dose of 250 mg/ kg showed a significant hyperglycemic effect.
• Yupparach, Piyapong & Konsue, Ampa. (2017). Hypoglycemic and Hypolipidemic Activities of Ethanolic Extract from Mimosa pudica L. in Normal and Streptozotocin-Induced Diabetic Rats. Pharmacognosy Journal. 9. 834- 837. 10. 5530/ pj. 2017. 6.130. Mimosa pudica L. its a unique property to collapses when touched and opens up. Aims: To evaluate hypoglycemic and hypolipidemic activities of 80% ethanolic extract from the whole plant of Mimosa pudica L. (MPE) by measuring fasting blood glucose in normal and streptozotocin (STZ)- induced diabetic rats treated with MPE. Materials and Methods: An eight-week study using MPE was performed in normal and streptozotocin (STZ)-induced diabetic rats. Hypoglycemic activities in normal and STZ-induced diabetic rats and oral glucose tolerance test (OGTT) and biochemical data including total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), and low-density lipoprotein (LDL) of MPE were compared with glibenclamide, a standard anti-diabetic drug. Results: OGTT showed that MPE did not decrease blood glucose both in normal and STZ-induced diabetic rats comparable to controls and glibenclamide-treated rats. Moreover, MPE did not affect FBG in the normal rats. However, it significantly (p> 0.05) decreased FBG in the diabetic rats while MPE increased HDL and decreased TC, TG, and LDL in the diabetic rats. Conclusions: The results from this study confirmed the traditional use of Mimosa pudica L. for the treatment of diabetes mellitus.
• Permatasari, Roselia & Pramyrtha Hestianah, Eka & Legowo, Djoko & Rachmawati, Kadek & Arifin, Zainal. (2022). The Effect of Mimosa Pudica Root Extract on Cerebrum Histopathology of Rattus Norvegicus Induced with Naja Sputatrix Venom. Journal of Basic Medical Veterinary. 11. 37- 48. 10. 20473/ jbmv. v11i1. 36485. The aim of this study was to know the effect of Mimosa pudica root extract on the histopathological appearance of Rattus norvegicus brain induced by Naja sputatrix venom. Thirty rats were divided into 5 groups. There were 2 control groups and 3 treatment groups, which were given 250, 500, and 1000 mg/ kg BW of Mimosa pudica root extract orally. For the first 7 days, each group was adapted to the environment. On the 8th day, the treatment was started by injecting Naja sputatrix LD50 (0,13 L/ gram BW) IM in gluteus muscle, continued with giving Mimosa pudica root extract orally for the treatment groups 5 minutes after venom injection. 6 hours after the last treatment, rats were killed by cervical dislocation, injected with formalin 10% in the heart, then necropsied. Histopathological evaluation was done to score brain damage based on meningitis, perivascular cuffing, and necrotic cells using HE stain with 1000x magnification. The result showed that 1000 mg/ kg BW dosage of Mimosa pudica root extract can reduce brain damage based on meningitis, perivascular cuffing, and necrotic cells in Rats (Rattus norvegicus) caused by Naja sputatrix venom and gave significant difference (p < 0.05) among the treatment groups.
• Paras, Helen & Tahir, Maham & Mehwish, Romana & Akhtar, Shabana & Tahir Maqbool, Dr & Chand, Yousaf & Qureshi, Javed & Hadi, Faheem & Atif, Muhammad & Mehdi, Prof. (2022). Hepatoprotective Effect of Mimosa pudica Leaves Ethanoic Extract in CCl4-Induced Hepatotoxicity. The aim of the current study was to evaluate the hepatoprotective potential of Mimosa pudica leaves ethanolic extract against carbon tetrachloride (CCl4)-induced hepatic damage in Wistar rats. Mimosa pudica is famous for its anticancer alkaloid, mimosine, along with several valuable secondary metabolites like tannins, steroids, flavonoids, triterpenes, and glycosyl flavones along with pharmacological properties like antioxidant, antibacterial, antifungal, anti-inflammatory, hepatoprotective, antinociceptive, anticonvulsant, antidepressant, antidiarrheal, hypolipidemic activities, diuretic, antiparasitic, antimalarial, and hypoglycemic have been attributed to different parts of Mimosa pudica. Administration of carbon tetrachloride in rats significantly increased the levels of biochemical parameters of liver damage; bilirubin, cholesterol, alanine transaminase (ALT), alkaline phosphate, albumin, aspartate transaminase (AST), urea, and creatinine. Test animals were subjected to different concentrations of ethanolic extract of Mimosa pudica leaves (500 mg/ kg. 750 mg/ kg and 1000 mg/ kg) through oral administration. Out of three doses, 500 mg/ kg showed better results in reducing the elevated levels of hepatic parameters and showed hepatocellular regeneration in CCl4-induced injured rats as compared with the CCl4 group alone. While higher doses (750 mg/ kg and 1000 mg/ kg) also showed significant results but less than 500 mg/ ml dose. Histopathological examination of the liver also indicated the protective effect of Mimosa pudica on injured liver tissues with improved architecture. The results of this study strongly suggest that mimosa pudica leaf extract has hepatoprotective and healing properties.
• Azam, Shofiul & Huda, Archi & Shams, Kishower & Ansari, Prawej & Mohamed, Khalid & Hasan, Mohammad Mahmudul & Azad, Abul & Mondal, Kallol & Zaouad, Shakil. (2015). Anti- Anti-Inflammatory and Antioxidant Study of Ethanolic Extract of Mimosa pudica. Journal of Young Pharmacists. 7. 234- 240. 10. 5530/ jyp. 2015. 3. 14. Objective: Our study includes the investigation of the phytochemical composition and in vitro free radical content and anti-inflammatory activity of Mimosa pudica. Materials and Method: A free radical scavenging assay was done to evaluate the dose-dependent reduction of free radicals by ethanolic extract of Mimosa pudica, this activity was compared with a reference antioxidant i.e. Ascorbic acid. The anti-inflammatory potential of ethanolic extract of Mimosa pudica has been determined by using carrageenan-induced paw edema assay and Cotton wool granuloma in rats. Results: The IC50 of our sample was 24.55 μg/ ml, it’s a very promising result compared to the same reference. At the dose of 300 mg/kg, the extract shows a considerable inhibitory effect on paw increase 1 hour after carrageenan administration, by inhibiting nearly 50%. The maximum inhibition (43.48 %, p< 0.001) elicited by the ethanolic extract was recorded 4 hours after carrageenan injection. Diclofenac sodium, which is a reference drug showed a similar inhibitory effect 4 hours after carrageenan administration (50.31%). The cotton wool granuloma is widely used to evaluate the transudative and proliferative components of chronic inflammation. Chronic inflammation occurs by means of the development of proliferated cells which can be spread in granuloma form. Non-steroidal anti-inflammatory drugs decrease the size of granuloma which results from cellular reaction by inhibiting granulocyte infiltration, preventing the generation of collagen fibers, and suppressing mucopolysaccharides. The extract showed significant (p< 0.01) anti-inflammatory activity in cotton wool-induced granuloma with 33.64 % inhibition at higher doses. Conclusion: This finding suggests that ethanolic extract of M. pudica possesses potent anti-inflammatory activity possibly due to its free radical scavenging properties.
• Ambikabothy, Jamunaa & Ibrahim, Halijah & Ambu, Stephen & Chakravarthi, Srikumar & Awang, Khalijah & Vejayan, Jaya. (2013). Efficacy evaluations of Mimosa pudica tannin isolate (MPT) for its anti-ophidian. Ambikabothy, Jamunaa & Ibrahim, Halijah & Ambu, Stephen & Chakravarthi, Srikumar & Awang, Khalijah & Vejayan, Jaya. (2011). Efficacy evaluations of Mimosa pudica tannin isolate (MPT) for its anti-ophidian properties. Journal of Ethnopharmacology. 137. 257- 62. 10. 1016/ j. jep. 2011. 05. 013. Evaluations of the anti-snake venom efficacy of Mimosa pudica tannin isolate (MPT) obtained from the root of the plant. MPT was investigated in vitro and in vivo for its efficacy against the venom of the Naja kaouthia snake. In vitro: (1) mice injected i. p. with MPT pre-incubated with Naja kaouthia venom at concentrations as low as 0.625 mg/ ml showed 100 % survival after a 24- h observation period. In the proteomics study, mice injected with MPT pre-incubated with the Naja kaouthia venom showed a down-regulation of five serum proteins. In the protein-dye-binding study, the percentage of Bradford dye-protein binding showed a reduction relative to the decrease in MPT concentration used to incubate with the venom. In vivo: the results from the animal studies showed that MPT had no in vivo protection against the Naja kaouthia venom (0.875 mg/ kg) in four different rescue modes and in an oral pre-treatment experiment. The study indicated the promising ability of MPT to neutralize the Naja kaouthia venom in vitro experiments but fell short in its in vivo potential. As such, the use of Mimosa pudica (Mimosaceae) as therapeutics for snake bites is questionable as all the possible in vivo rescue studies and pre-treatment of the active constituents showed no protection against the affected mice.
• Ngo Bum, Elisabeth & Dawack, D & Schmutz, M & Rakotonirina, A & Rakotonirina, S & Portet, C & Jeker, A & Olpe, H.-R & Herrling, Paul. (2004). Anticonvulsant activity of Mimosa pudica decoction. Fitoterapia. 75. 309- 14. 10. 1016/ j. fitote. 2004. 01. 012. The decoction of Mimosa pudica leaves given intraperitoneally at a dose of 1000-4000 mg/ kg protected mice against pentylenetetrazol and strychnine-induced seizures. M. pudica had no effect against picrotoxin-induced seizures. It also antagonized N-methyl-D-aspartate- induced turning behavior. These properties could explain its use in African traditional medicine.
• BASUMATARY, HIRINA & BORDOLOI, PALLAVI & DEKA, DIPJYOTI. (2024). EVALUATION OF ANTI-INFLAMMATORY ACTIVITY OF AQUEOUS EXTRACT OF MIMOSA PUDICA ON SWISS ALBINO MOUSE. International Journal of Pharmacy and Pharmaceutical Sciences. 12- 16. 10. 22159/ ijpps. 2024v16i8. 51414. Objective: To evaluate the acute anti-inflammatory activity of Aqueous Extract of Mimosa pudica (AEMP) on carrageenan-induced paw edema in Swiss albino mice and to compare the histopathology findings of control, standard, and treated Paws of mice. Methods: 24 Albino mice (20± 2g) were divided into 6 groups. Anti-inflammatory activity using the carrageenan-induced paw edema method was measured at various intervals on Day 1 followed by a histopathological examination of the paw. Aspirin was taken as the standard drug, and three different doses (100 mg/ kg, 200 mg/ kg, and 400 mg/ kg) of AEMP were taken as a test drug. The left paw of the mouse was considered as a control. Statistical analyses were done using Analysis of Variance (ANOVA) followed by Tukey’s multiple comparison tests, a p-value < 0.05 was considered for significant difference. Results: AEMP showed significant anti-inflammatory activity at all doses (100, 200, 400 mg/ kg) when compared to the carrageenan-induced group. The study showed that test drug AEMP at the dose of 400 mg/ kg produced a maximum reduction of paw edema at 4 h. The reduction in paw edema of mice was lower at 1 h and showed maximum reduction at 4 h. The AEMP significant (p≤ 0.05) anti-inflammatory activities in a dose-dependent manner to that of the standard drug Aspirin. Conclusion: The study suggests that AEMP has anti-inflammatory properties and can be used in the treatment of pain. However, further study is necessary in this regard.
• Prathima, C. & Shashikumara, & Thippeswamy, Thippeswamy & Jayanthi, M. K.  (2016). Evaluation of anticonvulsant activity of Mimosa pudica root linn in swiss albino mice. 8. 49- 52. 10. 22159/ ijpps. 2016. v8i9. 11716. Objective: To evaluate the anticonvulsant activity of ethanolic extract of Mimosa pudica root (EMPR) in experimental mice models. Methods: Ethanolic extract of root parts of Mimosa pudica (EMPR) was prepared by a continuous method using a soxhlet apparatus. EMPR in doses of 1000, 2000 mg/ kg body wt along with valproate were administrated to albino mice by oral route, and anti-epileptic activity was assessed by maximal electroshock (MES) and pentylenetetrazole (PTZ) induced seizure models. Abolition of the tonic hind limb extension phase and an increase in seizure latency period, when compared to the control group, was taken as a measure of protection in MES and PTZ-induced convulsion models respectively. Results: EMPR in the dose of 1000 and 2000 mg/ kg body wt. of mice showed significant anti-epileptic properties in both MES and PTZ-induced seizure models. There was a significant abolition of the tonic hind limb extension phase in the MES model. There was also a significant increase in seizure latency period in the induced seizure model. Conclusion: Results suggest that ethanolic extract of Mimosa pudica roots possess significant anti-epileptic activity. Further investigations are required to determine its active constituents and also its antiepileptic mechanism of action.
• Patro, Ganesh & Bhattamisra, Subrat & Mohanty, BijayKumar. (2015). Analgesic, antiepileptic, and behavioral study of Mimosa pudica (Linn.) on experimental rodents. International Journal of Nutrition, Pharmacology, Neurological Diseases. 5. 145. 10. 4103/ 2231- 0738. 167502. Objective: Mimosa pudica (M. pudica) Linn. (family: Mimosaceae) is a traditionally used folk medicine to treat various ailments including convulsion, alopecia, diarrhea, dysentery, insomnia, tumor, wounds, snake bites, etc., Here, the study was aimed to evaluate the potential on antiepileptic, analgesic, and motor activities of M. pudica leaves on rodents. Materials and Methods: In an acute toxicity study, the extracts were administered in doses of 50- 2,000 mg/ kg/ p.o., and behavioral changes were observed for up to 24 h. For a pharmacological study, the ethyl acetate extract of M. pudica (EAMP) leaves in doses of 100 mg/ kg/ day, 200 mg/ kg/ day, and 400 mg/ kg/ day were orally administered for consecutive 7 days to animals. The antiepileptic study was evaluated by inducing electric shock, pentylenetetrazole (PTZ), and isoniazid (INH) in mice, whereas the motor activity test was performed by using an actophotometer, rotarod test, and traction test in mice. The analgesic activity was done by hot-plate, tail flick, and acetic acid-induced writhing in rats. Statistical analysis was carried out by one-way analysis of variance (ANOVA) followed by Dunnett’s test. Results: The EAMP showed dose-dependent analgesic activity by increasing the reaction time as compared to the vehicle control. Similarly, the motor performance was improved in a dose-dependent manner as compared to the standard. The doses (100 mg/ kg/ day, 200 mg/ kg/ day, 400 mg/ kg/ day) of the extract significantly (P < 0.01 and P < 0.001) reduced the duration of seizures induced by maximal electroshock (MES) and delayed the onset of tonic-clonic seizures produced by PTZ and INH. All the tested doses significantly prevented the latency and duration of convulsion against seizure inducers as compared to the vehicle controls. Conclusion: These results revealed that the EAMP possesses potent analgesic, antiepileptic, and motor activities in animals. This could be an effective treatment option for various motor or seizure disorders. 
• Nair, Parvathy & Nair, Bindu. (2017). Anti-inflammatory activity of hydroalcoholic extract of mimosa pudica whole plant in rats. International Journal of Basic & Clinical Pharmacology. 10. 18203/ 2319- 2003. Ijbcp/ 20170473. Background: Mimosa pudica is a traditionally used folk medicine to treat various disorders like infections, anxiety, depression, bleeding disorders, convulsions, rheumatoid arthritis, muscular pain, asthma, snake bites, etc. We evaluated the anti-inflammatory activity of the hydroalcoholic extract of Mimosa pudica whole plant (HAEMPWP) in rats. Methods: HAEMPWP was prepared using Soxhlets apparatus. Acute toxicity tests were done with HAEMPWP given orally to albino rats in increasing doses up to 3200 mg/ kg body weight. The anti-inflammatory action was evaluated by the Carrageenan-induced paw edema method. Thirty albino rats were grouped into five groups, and each contained six rats. Group I (the control group) received distilled water orally. Group II (standard) received Aspirin orally dissolved in distilled water. Groups III, IV, and V received HAEMPWP in doses of 200 mg/ kg, 400 mg/ kg, and 800 mg/ kg orally dissolved in distilled water. Data analysis was done by one-way ANOVA and unpaired t-test using SPSS 16 for Windows.  HAEMPWP showed a significant anti-inflammatory activity as compared to the control. There was no statistically significant dose dependent increase in the anti-inflammatory activity. HAEMPWP possesses significant anti-inflammatory activity and could be an effective treatment option for various inflammatory conditions.
• Aiyelero, Oyeronke & Olatunde, K.O & Salawu, K. & Eniayewu, Oluwasegun & Fatimoh, Ojuade & Akinpelu, I. A & Mogaji, M.G. (2024). Phytochemical and Anticonvulsant Activity of the Ethanol Root Bark Extract of Mimosa pigra L. (Fabaceae) in Laboratory Animals. Nigerian Journal of Pharmaceutical Research. 20. 49- 55. 10. 4314/ njpr. v20i1. 6. Background and objectives: Various parts of Mimosa pigra (MPG) are used in traditional medicines to treat convulsive disorders. The objective of this study was to investigate the anticonvulsant properties of Mimosa pigra ethanol root extract (EREM). Methods: The acute toxicity of the extract was investigated using the OECD 423 protocol of 2002. The anticonvulsant properties of EREM at 200,400 and 800 mg/ kg were evaluated using Maximal Electroshock Test (MEST) in chicks; strychnine (SCN-) and pentylenetetrazole (PTZ)-induced seizures in mice. Results: The extract at 400 and 800 mg/ kg significantly (p< 0.05) prolonged the mean onset of clonic and tonic convulsions in a mouse model of SCN-induced seizure. In PTZ-induced seizure, the extract at 400 mg/ kg significantly (p< 0.05) increased the mean onset of clonic seizure, while at 800 mg/ kg, there was significant (p< 0.05) prolongation in the mean onset of clonic and tonic seizure compared to control. The extract did not protect the chick against MEST but significantly (p < 0.05) reduced the mean recovery time at 200, 400, and 800 mg/ kg. The extract offered 60 and 100 % protection at 400 and 800 mg/ kg respectively in SCN-induced seizure. Similarly, EREM offered 20 and 40 % protection at 400 and 800 mg/ kg respectively in PTZ-induced seizures. Diazepam (10 mg/ kg), a reference drug significantly (p< 0.05) prolonged the onset of a clonic-tonic seizure and protected against SCN-, and PTZ-induced convulsion in mice. Conclusion: These findings indicated that EREM may possess anticonvulsant activity in SCN-, and PTZ-induced seizure in mice. Thus, lend scientific credence to the anticonvulsant claim of EREM in ethnomedicine.
• Ganguly, Mausumi & Devi, Nirada & Mahanta, Rita & Borthakur, Mridul Kumar. (2008). Effect of Mimosa pudica root extract on vaginal estrous and serum hormones for screening of antifertility activity in albino mice. Contraception. 76. 482- 5. 10. 1016/ j. contraception. 2007. 08. 008. Several plants are traditionally used as birth control agents by rural people in India. Mimosa pudica is one of the folk medicinal plants commonly used as an antifertility agent in some places in India. The present work was carried out to evaluate the claimed antifertility effect of the plant by carrying out pharmacological studies with the root extract of the plant.
• Air-dried roots of M. pudica were extracted using methanol. Dried methanol extract of the root was administered orally to Swiss albino mice for 21 consecutive days. Estrous cycle, reproductive hormones (LH, FSH, prolactin, estradiol, and progesterone), and number of litters produced were studied in both control and extract-administered groups by using standard methods. Phytochemical studies of the methanolic root extract were carried out using qualitative and thin-layer chromatography methods. M. pudica root extract, when administered orally at a dose of 300 mg/ kg body weight/ day, prolonged the length of the estrous cycle with a significant increase in the duration of the diestrus phase and reduced the number of litters in albino mice. The number of litters increased in the posttreatment period. The analysis of the principal hormones (LH, FSH, prolactin, estradiol, and progesterone) involved in the regulation of the estrous cycle showed that the root extract altered gonadotropin release and estradiol secretion. The root extract of M. pudica has an anti-fertility effect as it prolongs the estrous cycle and disturbs the secretion of gonadotropin hormones in albino mice. The decrease in FSH levels in the proestrus and estrus stages in the extract-administered group compared with those of control animals indicates the disturbance of the estrous cycle and ovulation through suppression of FSH.
• Ranjan, R.K. & Manoharan, Sathish Kumar & Seethalakshmi, I. & Ram Krishna Rao, Mudiganti. (2013). Phytochemical analysis of leaves and roots of mimosa pudica collected from Kalingavaram, Tamil Nadu. Journal of Chemical and Pharmaceutical Research. 5. 53- 55. Mimosa pudica is used in diseases related to blood and bile, bilious fever, piles, jaundice, leprosy, ulcers, and smallpox. In the present study, ethanolic extracts of Mimosa pudica leaves and root samples were obtained using a soxhlet apparatus. Phytochemical studies for the presence of revealed that tannin and proteins are present in both samples.
• Valsala, S & Karpagaganapathy, P. (2002). Effect of Mimosa pudica root powder on estrous cycle and ovulation in cycling female albino rat, Rattus norvegicus. Phytotherapy research: PTR. 16. 190- 2. 10. 1002/ ptr. 924. Mimosa pudica root powder (150 mg/ kg body weight) when administered intragastrically, altered the estrous cycle pattern in female Rattus norvegicus. Nucleated and cornified cells were absent in all rats. The smear was characterized by leucocytes only, as in dioestrus, which persisted for 2 weeks. There was a significant reduction in the number of normal ova in rats treated with the root powder compared with the control rats and a significant increase in the number of degenerated ova. 
• Munasinghe, Anuruddha & EDC, Karunarathna & ADH, Sudesh & Manohara, Mattaka Gamage & YMTM, Wijethunga. (2024). Antibacterial Activity of Aqueous Extract of Mimosa Pudica L. against Selected Wound Infecting Bacteria. International Journal of Research Publication and Reviews. 5. 5068- 5072. Aqueous extract of Mimosa pudica is utilized by certain Sri Lankans as a wound-cleaning agent. The present investigation was conducted to detect the invitro antibacterial activity of an aqueous extract of Mimosa pudica qualitatively against selected four wound infecting bacteria with agar dilution method at Gampaha Wickramarachchi Ayurveda University, Sri Lanka. Washed and Shade dried whole plant of Mimosa pudica was boiled with distilled water for 15 minutes and the cold extract was filtered with gauze to prepare the aqueous extract (neat solution). The neat solution as well as a serial dilution of it up to a quarter were used with Mueller-Hinton agar separately to prepare a series of culture plates. Wounds infecting pathogens such as Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Methicillin-resistant Staphylococcus aureus (MRSA) were inoculated separately into the culture plates. Plates were incubated overnight at 37 °C. For the negative control, plates were prepared with distilled water and Mueller-Hinton agar in place of plant extract. For the positive control, the extract was replaced with ampicillin. Except for Escherichia coli, all the other bacteria were sensitive to the extract. Pseudomonas aeruginosa was sensitive only to the neat solution. The other two strains were sensitive even in the half-neat solution. Thus, the aqueous extract of Mimosa pudica has an antibacterial effect on tested bacteria including MRSA. On outcome, scientists may launch further investigations on the plant for harnessing the natural resources effectively for universal health.
• Mandal, Ashok Kumar & Pandey, Anisha & Sah, Ranjit & Baral, Adesh & Sah, Phoolgen. (2022). In Vitro Antioxidant and Antimicrobial Potency of Mimosa pudica of Nepalese Terai Region: Insight into L- Mimosine as an Antibacterial Agent. Evidence-Based Complementary and Alternative Medicine. 2022. 1- 11. 10. 1155/ 2022/ 6790314. The study aimed to evaluate the in vitro antioxidant and antimicrobial potency of Mimosa pudica found wildly in the Terai region of Nepal and assess its physicochemical properties, such as total phenolic content (TPC) and total flavonoid content (TFC). The physicochemical properties of ethyl acetate extract of Mimosa pudica (EAMP), such as extractive value, total ash content, loss on drying, and phytochemical screening, were calculated using standard protocols. The TPC was determined by using the Folin-Ciocalteu method taking gallic acid as standard, and TFC was conducted by using the AlCl3 colorimetric method, using a 96-well plate reader. The in vitro antibacterial activity of different concentrations of the extract against four bacterial ATCC strains was determined by the agar well diffusion method in the Mueller Hinton agar (MHA) medium. The in silico molecular docking model was used to ascertain the antibacterial potency of L- L-mimosine against the selected strains of bacteria used for the in vitro study by calculating the binding affinity towards the protein of bacteria. The preliminary screening of the extract showed the presence of several phytochemicals. The total ash content (7.67 %), loss on drying (2.30 %), and extractive value (8.966 %) were determined by analyzing the crude sample. The total phenolic and flavonoid contents were 418.640 ± 0.018 mg GAE/g (dried extract) and 14.126 ± 0.021 mg QE/ g (dried extract), respectively. The extract showed a potent free radical scavenging activity with an IC50 value of 158.95 ± 1.12 µg/ mL. The plant extract also demonstrated antibacterial activity against both Gram-positive bacteria Staphylococcus aureus (15 mm) and Bacillus cereus (22 mm) and Gram-negative bacteria Escherichia coli (17 mm) and Klebsiella pneumoniae (16 mm) at 200 mg/ mL concentration of extract. There was a noteworthy binding affinity of antibiotics with almost all selected bacterial proteins with binding energy against Escherichia coli DNA gyrase subunit B (-5.7 kcal/ mol), Staphylococcus aureus DNA gyrase subunit B (-6.1 kcal/ mol), Bacillus cereus metallation transferase (-5.2 kcal/ mol), and Klebsiella pneumoniaebeta-lactamase (-6.1 kcal/mole), respectively, with the L- mimosine. The findings of the current study suggest that Mimosa pudica from the Terai region of Nepal is rich in phenolic and flavonoid compounds, has a significant impact on bacterial growth inhibition, and has a notable potential to scavenge free radicals (DPPH). According to the in-silico analysis, L- mimosine is a potent antibacterial compound that might be utilized to discover novel antibacterial drugs to combat antibiotic resistance.
• Nair, Parvathy & Nair, Bindu & Sasidharan Palappallil, Dhanya. (2023). Evaluation of diuretic activity of hydroalcoholic extract of whole plant Mimosa pudica. Journal of Cardiovascular Disease Research. 14. 1203- 1207. 10. 31838/ jcdr. 2023. 14. 06.154. Introduction: Diuretics are required for various pathological conditions with fluid overload. Our study has evaluated the diuretic activity of hydroalcoholic extract of the whole plant Mimosa pudica (HAEWMP) as an alternative/new drug that may induce diuresis. Materials and Methods: HAEWMP was prepared using Soxhlet’s apparatus. Albino rats were divided into 5 groups of 6 rats each. Group I (Control) received distilled water 25 ml/ kg orally. Group II (Standard) received Hydrochlorothiazide 2.5 mg/ kg orally, Group III, IV, and V 200 mg/ kg, 400 mg/ kg, and 800 mg/ kg. The urine samples were collected for all the groups at 5 and 24 hours and urine volumes were measured. The urinary excretion percentage, diuretic activity, and electrolytes (Na+, K+) were estimated. Results: HAEWMP exhibited significant dose-dependent diuretic activity by increasing urine volume and also by enhancing the elimination of Sodium (Na+) and potassium (K+). The diuretic activity of 200 mg/ kg and 400 mg/ kg was moderate and that of 800 mg/ kg was good. Conclusion: HAEWMP possesses significant diuretic activity and has a beneficial role in a volume overload condition
• Rajendran, Rekha & Hemalatha, S & Akasakalai, K & Madhukrishna, C & Sohil, Bavan & Sundaram R, Meenakshi. (2009). Hepatoprotective activity of Mimosa pudica leaves against Carbon tetrachloride-induced toxicity. J Nat Prod. 2. The methanolic extract of leaves of Mimosa pudica at the dose of 200 mg/ kg body weight per oral was studied for the hepatoprotective effect using Carbontetrachloride-induced liver damage in Wistar albino rats. Methanolic extract showed a significant (p< 0.05) hepatoprotective effect by lowering the serum levels of various biochemical parameters such as serum glutamic oxaloacetate transaminase (SGOT), serum glutamic pyruvates transaminase (SGPT), alkaline phosphatase (ALP), total bilirubin (TBL), total cholesterol (CHL) and by increasing the levels of total protein (TPTN) and albumin (ALB), in the selected model. These biochemical observations were in turn confirmed by histopathological examinations of liver sections and are comparable with the standard hepatoprotective drug Silymarin (100 mg/ kg body weight i.p.) which served as a positive control. The overall experimental results suggest that the biologically active phytoconstituents such as flavonoids, glycosides alkaloids present in the methanolic extract of the plant Mimosa pudica, may be responsible for the significant hepatoprotective activity and the results justify the use of Mimosa pudica as a hepatoprotective agent. Mimosa pudica (Mimosaceae) known as the Mue, is a stout struggling prostrate shrubby plant with compound leaves that gets sensitive to touching, spinous stipules, and globose pinkish flower heads, grows as a weed in almost all parts of the country (Ghani, 2003). Leaves and stems of the plant have been reported to contain an alkaloid mimosine, leaves also contain mucilage and roots contain tannins (Ghani, 2003). Mimosa pudica is used for its anti-hyperglycemic (Uma Maheswari, 2007), diarrhoeal (Balakrishnan, et al., 2006), anti-convulsant (Bum, et al., 2004), and cytotoxic properties (Sadia Afreen Chowdhury, et al., 2008).
• Aziz, Uddipon & Akhter, Rumana & Shahriar, Mohammad & Bhuiyan, Mohiuddin. (2014). In vivo pharmacological investigation of Mimosa pudica L. International Journal of Pharmacy and Pharmaceutical Sciences. 6. 66- 69. Objective: To evaluate the anti-nociceptive, acute toxicity, gastrointestinal motility, and anti-pyretic investigations of leaf extract of Mimosa pudica L. leaves in Swiss albino mice following oral administration. Methods: In-vivo anti-nociceptive activity test was evaluated by tail immersion test. In-vivo acute toxicity test was conducted using the acute toxic class method. In-vivo gastrointestinal motility was determined by charcoal feces defecation time. In-vivo antipyretic activity test was evaluated by brewer’s yeast-induced pyrexia. Results: In-vivo anti-nociceptive activity test shows that methanol & ethanol extracts (250 & 500 mg/ kg b. w.) performed significant activity (p< 0.05) in mice compared to the standard drug diclofenac Na. In-vivo acute toxicity test was done on mice with methanol, ethanol, and chloroform extracts (2000, 1000, 500 mg /kg b. w.) of Mimosa pudica leaf and no reaction or death occurred in mice during two weeks of observation. In-vivo gastrointestinal motility test indicates a significant (p< 0.01) increase in gastrointestinal motility by ethanol extracts of (250 & 500 mg/ kg b. w.) compared to the standard drug loperamide. In-vivo antipyretic activity test shows that methanol (250 & 500 mg/ kg b. w.), ethanol (250 & 500 mg/ kg b. w.), and chloroform (250 mg/ kg b. w.) extracts showed significant (p< 0.05) reduction in the temperature of mice compared to the standard drug paracetamol. The result of the study indicates analgesic and antipyretic properties along with gastrointestinal motility-stimulating effects. According to the acute toxicity study, leaf extracts are safe up to 2000 mg/ kg in-vivo concentration.
• Rajendran, Rekha. (2010). Diuretic and laxative activities of methanol extract of Mimosa pudica L. leaves. Medicinal Plants – International Journal of Phytomedicines and Related Industries. 2. 237. 10. 5958/ j. 0975- 4261. 2. 3. 038. The methanol extract of leaves of Mimosa pudica L. (Mimosaceae) was screened for diuretic and laxative activities in Wistar albino rats. The study suggested that the extract was found to produce significant diuretic as well as laxative activities in dose dose-dependent manner (200 and 400 mg/ kg p.o.). These activities were comparable with the standard drugs such as Furosemide (10 mg/ kg p.o.) and Agar-agar (300 mg/ kg p.o.) respectively. The preliminary phytochemical analysis of methanol extract from leaves of Mimosa pudica revealed the presence of phytoconstituents such as alkaloids, tannins, mucilage, and flavonoids. The present study indicates that the observed significant diuretic and laxative activities of Mimosa pudica leaves may be attributed to the phytoconstituents present in them.
• Baghel, A & Rathore, Devendra & Gupta, Vatsal. (2013). Evaluation of Diuretic Activity of Different Extracts of Mimosa pudica Linn. Pakistan journal of biological sciences: PJBS. 16. 1223- 5. 10. 3923/ pjbs. 2013.1223.1225. In that study, Mimosa pudica linn was tested for diuretic activity using the Lipschitz test. The ethanolic and aqueous extract of Mimosa pudica Linn. was studied at two dose levels 100 and 200 mg kg (-1) b. wt. Furosemide (20 mg kg (-1) b. wt.) was used as a standard drug in a 0.9 % saline solution. Urine volumes were measured for all the groups up to 5 h. The ethanolic extract of Mimosa pudica linn exhibited significant diuretic activity at doses of 100 and 200 mg kg (-1) b. wt. by increasing total urine volume and ion concentration of Na+ k+ and Cl-.
• Kalabharathi, H.L. & Shruthi, S.L. & Vaibhavi, P.S. & Vh, Pushpa & Satish, A.M. & Sibgatullah, Mohammed. (2015). Diuretic Activity of Ethanolic Root Extract of Mimosa Pudica in Albino Rats. Journal of clinical and diagnostic research: JCDR. 9. FF05- FF07. 10. 7860/ 2014/ 14662. 6877. Diuretics are the drugs that increase the urine output. This property is useful in various pathological conditions of fluid overload. The presently available diuretics have a lot of adverse effects. Our study has evaluated the diuretic activity of ethanolic root extract of Mimosa pudica as an alternative/new drug that may induce diuresis. To evaluate the diuretic activity of ethanolic root extract of Mimosa pudicaa in albino rats. The ethanolic root extract of Mimosa pudica (EEMP) was prepared using Soxhlet’s apparatus. Albino rats were divided into 5 groups of 6 rats each. Group- I (Control) received distilled water 25 ml/ kg orally. Group II (Standard) received Furosemide 20 mg/ kg orally. Group- III received EEMP 100 mg/ kg, Group- IV received EEMP 200 mg/ kg and Group- V received EEMP 400 mg/kg. The urine samples were collected for all the groups up to 5 hours after dosing and urine volume was measured. Urine was analyzed for electrolytes (Na+, K+, and Cl-). ANOVA, Dunnet’s test, and p-values were measured, and data was analyzed. EEMP exhibited significant diuretic activity by increasing urine volume and also by enhancing the elimination of Sodium (Na+), Potassium (K+), and Chloride (Cl-) at doses of 100 and 200 mg/ kg. EEMP possesses significant diuretic activity and has a beneficial role in volume overload conditions.